The fluorescent labels of AquiStain are synthetic analogues of epicocconone, small naturally occurring fluorescent compound, marketed by Fluorotechnics as LavaPurple ™ or DeepPurple. ™
This technology is of great interest in the field of life sciences. Indeed, fluorescent compounds are widely used for diagnostic purposes, for demonstrating enzymatic activity and for visualizing proteins in biological media or in aqueous solutions, such as the 1D and 2D gel electrophoresis.
Just as epicocconone, our fluorescent labels AquiStain reversibly bind to lysine, arginine and histidine residues in proteins and peptides to yield an intensely red fluorescent product for identifying and quantifying these residues.
With its efficient synthesis process, AquiStain quickly develops fluorescent labels with high purity in a sustainable way.
AquiStain’s labels properties:
• Very sensitive, superior efficiency to Sypro® Ruby or silver staining.
• Ideal for multiplexing with other fluorophores
• A very large Stocks shift of about 100 nm, a small size and low molecular weight
• A good solubility in both water and in lipid bi-layers
• For use in living systems to measure dynamic processes in real time
• Compatible with standard instrumentation for fluorescence
• Environmentally friendly, heavy metal free and non-toxic.
The AquiStain fluorescent labels are the result of innovative technology and know-how patented.
The different studies of these compounds have already resulted in the publication of many scientific articles.
• Characterization of Fluorescent Synthetic Epicocconone-based Dye through Advanced Light Microscopies for Live Cell Imaging Applications, Shapman, D.; Perraudeau, C.; Bénard, M.; Gallavardin, T.; Boulangé, A.; Leleu, S.; Lebon, A.; Franck, X.; Galas, L. Dyes and Pigments 2017, 141, 394-405.
• Epicocconone-Hemicyanine Hybrids: Near Infrared Fluorophores for Protein Staining and Cell Imaging, Karuso P.; Loa Kum Cheung, W.; Peixoto, P.A.; Boulangé, A.; Franck, X. Chem. Eur. J. 2017, 23, 1820-1829.
• Design and synthesis of epicocconone analogues with improved fluorescence properties, Peixoto, P.A.; Boulangé, A.; Ball, M.; Naudin, B.; Alle, T.; Cosette, P.; Karuso, P.; Franck, X. J. Am. Chem. Soc, 2014, 136, 15248-15256.
• Evaluating charge transfer in epicocconone analogues: Toward a targeted design of fluorophores, Syzgantseva, O.A.; Tognetti, V.; Boulangé, A.; Peixoto, P.A.; Leleu, S.; Franck, X.; Joubert, L. J. Phys. Chem. A 2014, 118, 757-764.
• The role of different structural motifs in the ultrafast dynamics of second generation protein stains, Chatterjee, S.; Karuso, P.; Boulangé, A.; Peixoto, P.A.; Franck, X.; Datta, A. J. Phys. Chem. B 2013, 117, 14951-14959.
• Electronic Excitations in Epicocconone Analogues: TDDFT Methodological Assessment Guided by Experiment, Syzgantseva, O.A.; Tognetti, V.; Joubert, L.; Boulangé, A.; Peixoto, P.A.; Leleu, S.; Franck, X. J. Phys. Chem. A 2012, 116, 8634-8643.
• Diastereoselective IBX oxidative dearomatization of phenols by remote induction: Towards the epicocconone core framework, Boulangé, A.; Peixoto, P. A.; Franck, X. Chem. Eur. J. 2011, 17, 10241.
To increase and enhance its technology and its applications, AquiStain carries its own internal R & D, but also has partnerships with academic researchers and pharmaceutical companies.
To protect but also protect customers, AquiStain works under confidentiality agreement for all of its collaborations.
AquiStain thank all its partners for their confidence.